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杨江科
来源:武汉工业学院生物与制药工程学院    时间:2012/09/07    编辑:    点击:

杨江科,博士,教授。湖北省楚天学者计划楚天学子岗位。

2011年5月至今,武汉轻工大学。组建了工业酶分子改造与生物转化实验室。系统开展了工业酶基因资源的深度挖掘、分子改造、高效表达以及产业化示范等工作;并开展以工业酶为催化剂的绿色生物转化关键技术研究工作。目前共发表文章80余篇。其中SCI和EI收录论文20余篇。

2009年2月-2011年8月,在香港科技大学生命科学部从事研究。为香港生物技术学会会员。主要承担了香港科技大学(HKUST)与沙特阿拉伯阿卜杜拉国王科技大学(KAUST)合作项目:红海微生物宏基因组学和天然代谢产物的研究(SA-C0040/UK-C0016)。通过宏基因组学手段,系统挖掘了深海微生物资源,新型的重要功能基因和重要代谢途径,为新型工业酶的应用提供了丰富的基因资源。

2003年-2009年2月,华中科技大学生命科学与技术学院从事教学和科研工作。

2005年3月至2005年10月,受欧盟(FP6)玛丽.居里基金资助,在以色列魏兹曼研究院生物化学系从事研究。

2002年至2003年,受法国Miistere de la jeunesse,de l’Education Nationale et de la Recherche资助,在法国Perpignan大学基因组与发育实验室(CNRS5096)从事博士后研究。

1999年-2002年,华中农业大学生命科学技术学院,获微生物学理学博士学位。

1996年至1999年,华中农业大学生命科学技术学院,获微生物学理学硕士学位。

近五年主持或参加的国家级重大科研项目以及所获各种人才计划或基金的资助情况如下:

国家自然基金项目,基于新型邻苯二酚-2,3-双加氧酶基因芘降解假单胞菌的同源重构与时空变化分析(31460027)(子项目),时间:20015-2018年,主持。

湖北省支撑计划项目, 地方优势饲料资源的开发与高效利用(子项目),时间:20014-2016年,主持。

教育部科学研究重点项目,基于环境微生物宏基因组数据库新型脂肪酶基因资源的挖掘及分子改造(编号:212118) 时间:2012-2014年,主持。

湖北省教育厅重点项目,基于二步法全基因合成技术饲用耐高温脂肪酶的分子改造(D20131703),时间:2012-2014年,主持。

主持“十一五”先进能源领域863项目:生物柴油新型脂肪及工艺研究(2007AA05Z217),总经费:80万元,时间:2007-2010年,主持。

主持“十一五”863重点项目:生物柴油生产用绿色合成催化材料技术,子课题(2009AA03Z232),总经费:45万元,时间:2009-2010年,主持。

主要的研究论文:

1. Zhou WJ, Yang JK∗, Mao L, Miao LH. Codon optimization, promoter and expression system selection thatachieved high-level production of Yarrowia lipolytica lipase in Pichia pastoris. Enzyme and Microbial Technology 2015,71:66–72

2. Yang JK∗, Xiong W, Xu L, Li J, Zhao XJ. Constitutive expression of Campylobacter jejuni truncated hemoglobinCtrHb improves the growth of Escherichia coli cell under aerobic andanaerobic conditions. Enzyme and Microbial Technology 2015, 75–76:64–70

3. Xu L, Xiong W, Yang JK, Li J, Tao XW. Recombinant Escherichia coli strains with inducible Campylobacter jejuni single domain hemoglobin CHb expression exhibited improved cell growth in bioreactor culture. PLoS ONE. 2015, 10(3): e0116503.

4. Yang JK, Zhang JJ, Yu HY, Cheng JW, Miao LH. Community composition and cellulase activity of cellulolytic bacteria from forest soils planted with broad-leaved deciduous and evergreen trees. Appl Microbiol Biotechnol. 2014, 98(3):1449-1458.

5. Yang JK, Cheng ZB, Li J, Miao LH, Community Composition of NirS-Type Denitrifier in a Shallow Eutrophic Lake, Microbial Ecol, 2013, 66(4):796-805.

6. Yang JK, Liu LY, Dai JH, Li Q. de novo design and synthesis of Candida antarctica Lipase B gene and α-factor leads to high-level expression in Pichia pastoris. PLoS ONE. 2013, 8(1): e53939.

7. Yang JK, Chen FYuan, Yan XX, Miao LH, Dai JH. A simple and accurate two-step long DNA sequences synthesis strategy to improve heterologous gene expression in Pichia. PLoS ONE, 2012, 7(5): e36607.

8. Yang JK, Sun J, Lee OO, Wong YH, Qian PY. Phylogenetic diversity and community structures of sponge-associated bacteria from the mangroves of the Caribbean Sea. Aquatic Microbiology Ecology, 2011, 62: 231-240.

9. Bougouffa S., Yang J. K, Lee O. O., Wang Y., Batang Zenon, Al-Suwailem A. Qian P. Y. Distinctive microbial community structure in highly stratified deep-sea brine water column. 2013, doi: 10.1128/ AEM.00254-13

10. Lee OO, Yang J, Bougouffa S, Wang Y, Batang Z, Tian R, Al-Suwailem A, Qian PY. Spatial and species variations in bacterial communities associated with corals from the Red Sea as revealed by pyrosequencing. Appl Environ Microbiol. 2012, 78(20):7173-7184.

11. Yang J, Sun J, Lee O O, Wong Y H, Qian P Y. Phylogenetic diversity and community structures of sponge-associated bacteria from the mangroves of the Caribbean Sea. Aquatic Microbiology Ecology, 2011, 62: 231-240. (IF=1.7)

12. Wang Y, Yang J, Lee O O, Dash S, Lau S C K, Al-Suwailem A, Wong TYH, Danchin A, Qian PY. Hydrothermally generated aromatic compounds are consumed by bacteria colonizing in Atlantis II Deep of the Red Sea. The ISME Journal (in press) (IF=6.4)

13. Lee OO, Wang Y, Yang J, Lafi FF, Al-Suwailem A, Qian PY. Pyrosequencing reveals highly diverse and species-specific microbial communities in sponges from the Red Sea. ISME J. 2011, 5(4):650-664.

14. Pei-Yuan Qian, Yong Wang, On On Lee, Jiangke Yang, Stanley Lau,Abdulaziz Al-Suwailem, Tim Wong. Vertical stratification of microbial communities in deep-sea brine pools and overlying water columns in the Red Sea. The ISME Journal, 2011, 5: 507–518. (IF=6.4)

15. Yang J, Sun J, et al. lip2, a novel lipase gene cloned from Aspergillus niger exhibits enzymatic characteristics distinct from its previously identified family member. Biotechnology Letter, 2010, 32(7): 951-956. (IF=1.6)

16. Yang J, Liu L, Cao X, Combination of bioimprinting and silane precursor alkyls improved the activity of sol–gel-encapsulated lipase. Enzyme and Microbial Technology 2009, 46 (3): 257-261. (IF=2.6)

17. Yang J, Liu L. Codon optimization through a two-step gene synthesis leads to a high-level expression of Aspergillus niger lip2 gene in Pichia pastoris. Journal of Molecular Catalysis B: Enzymatic, 2010, 63 (3): 164-169. (IF=2.4)

18. Xu L*, Jiang X*, Yang J*, et al. Cloning of a novel lipase gene, lipJ08, from Candida rugosa and expression in Pichia pastoris by codon optimization Biotechnol Lett. 2010, 32(2):269-276.(*Equal contribution) (IF=1.6)

19. Jia B, Yang JK, et al. Homologous overexpression of a lipase from Burkholderia cepacia using the lambda red recombinase system. Biotechnol Lett. 2010, 32(4): 521-526. (IF=1.6)

20. Shu Z, Duan M, Yang J, et al. Aspergillus niger lipase: heterologous expression in Pichia pastoris, molecular modeling prediction and the importance of the hinge domains at both sides of the lid domain to interfacial activation. Biotechnology Progress, 2009, 25(2): 409 – 416. (IF=2.4)

21. Yang J, Zhang B, et al. Cloning and expression of Pseudomonas fluorescens 26-2 lipase gene in Pichia pastoris and characterizing for transesterification. Applied Biochemistry and Biotechnology, 2009, 159(2): 355-365. (IF=1.4)

22. Cao X, Yang J*, et al. Improving esterification activity of Burkholderia cepacia lipase encapsulated in silica by bioimprinting with substrate analogues, Process Biochem 2009, 44, 177–182.(*Correspondence author) (IF=2.4)

23. Yan JY, Yan YJ, Yang JK, et al. Combined strategy for preparation of a bioimprinted Geotrichum sp. lipase biocatalyst effective in non-aqueous media. Process Biochemistry, 2009, 44, 1128-1132. (IF=2.4)

24. Yang J K, Zhou J C. Diversity, phylogeny and host specificity of soybean and peanut bradyrhizobia. Biol Fertil Soils, 2008, 44:843–851 (IF=1.7)

25. Yang J K, Yuan T Y, Zhang W T, Zhou J C, Li Y G. Polyphasic characterization of mung bean (Vigna radiata L.) rhizobia from different geographical regions of China. Soil Biology & Biochemistry 2008, 40: 1681–1688. (IF=3.0)

26. Yang JK, Guo D, Zhou W, et al. Cloning, expression and characterization of a novel thermal stable and short-chain alcohol tolerant lipase from Burkholderia cepacia strain G63. Journal of Molecular Catalysis B: Enzymatic, 2007, 45: 91-96. (IF=2.4)

27. Yan J, Yang J, Xu L, et al. Gene cloning, overexpression and characterization of a promising lipase from Galactomyces geotrichum Y05. Journal of Molecular Catalysis B: Enzymatic. 2007, 49:28–35. (IF=2.4)

28. Yang J K, Zhang W T, Yuan T Y, Zhou J C. Genotypic characteristics of the rrn operon and genome of indigenous soybean Bradyrhizobia in cropping zones of China, Can, J. Microbiol. 2006, 52: 968-976. (IF=1.3)

29. Yang J K, Xie F L, Zou J, Zhou Q, Zhou J C. Polyphasic characteristics of bradyrhizobia isolated from nodules of peanut (Arachis hypogaea) in China. Soil Biology & Biochemistry, 2005, 37: 141–153 (IF=3.0)

30. Yang J K, Zhou J C. Diversity and phylogeny of Bradyrhizobium sp Arachis isolated from nodules of peanut in China. Source: Biological nitrogen fixation, sustainable agriculture and the environment. Book Series: Current Plant Science and Biotechnology in Agriculture. Volume: 41: 411-411 Published: 2005.Editor(s): Wang YP, Lin M, Tian ZX, Elmerich C, Newton WE.

 

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